Fig. 3
Comparison of the inner ear cell tropism of four AAV serotypes at early mature stage (P14-P18). Confocal images of the apical turn of the organ of Corti (left panels) and vestibular organs (right panels) after injection through the RWM at P14-18 with 2 µL of (A) AAV2-CBA-GFP, (B) AAV8-CMV-GFP, (C) AAV-Anc80L65-CMV-GFP or (D) AAV9-PHP.eB-CBA-GFP, followed by immunostaining, at P19-25, for GFP (green) and myosin 7a (red). For the cochlea (left), the insets provide higher-magnification views of the apical (top), medial (middle), and basal (bottom) regions (scale bars: A–D, 100 μm; insets, 5 μm). IHCs, inner hair cells; OHCs, outer hair cells; SGNs, spiral ganglion neurons. For the vestibule (right), broad view of the macula and ampulla cristae with corresponding close-up views (scale bars A–D, 50 μm; insets, 5 μm). GFP-positive vestibular hair cells are outlined by dashed lines and cells other than sensory hair cells are indicated by arrowheads. (E) Bar graphs showing the percentage of hair cells—IHCs, OHCs and VHCs—transduced with AAV2 (black), AAV8 (pink), Anc80L65 (blue), or AAV9-PHP.eB (purple), (mean percentage ± SEM). Two-way ANOVA, **P < 0.01, ***P < 0.001 and ****P < 0.0001.
