Fig. 4
Comparison of the inner ear tropism of four AAV serotypes at late mature stage (P30). Confocal images of the apical turn of the organ of Corti (left panels) and vestibular organs (right panels) injected at P30 with 2 µl of (A) AAV2-CBA-GFP, (B) AAV8-CMV-GFP, (C) AAV-Anc80L65-CMV-GFP and (D) AAV9-PHP.eB-CBA-GFP via RWM, immunostained at P37 for GFP (green) and myosin 7a (red). For the cochlea, higher magnification insets of the apical (top), medial (middle), and basal (bottom) regions are inserted (scale bars: A-D, 100 μm; insets, 10 μm). On the right panel, large view of the macula and ampulla cristae with corresponding higher magnification (scale bars A-D, 50 μm; insets, 10 μm). (E) Comparative analyses of the transduction rate of inner hair cells (IHCs), outer hair cells (OHCs) and vestibular hair cells (VHCs) transduced with different serotypes including AAV2 (black), AAV8 (pink), Anc80L65 (blue), and AAV9-PHP.eB (purple), (mean percentage ± SEM). Two-way ANOVA, *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001.
