Fig. 3
From: Improved acid-driven inhibition of effector T cell function by a pHLIP variant-conjugated PD-L1
Validation of the binding of PD-L1-pHLIPva to anti-PD-L1 mAb and PD-1 protein. (a) Schematic illustration of assembly of pHLIPva-conjugated PD-L1. (b) The binding capacity of PD-L1-pHLIPva as well as PD-L1-pHLIPwt to an anti-murine PD-L1 antibody was determined by ELISA. (c) The ability of PD-L1-pHLIPva as well as PD-L1-pHLIPwt to bind recombinant murine PD-1 protein was determined by ELISA. The data was pooled from three experiments.
