Table 2 Chemical composition of FSM/DNA, FHS/DNA, FSMPF/DNA, and FHSPF/DNA NPs; with schematic illustration of preparation.
From: A novel approach in using insect-based spinach-food waste for gene targeting to cancer tissues
MNPs ID | FSM (µg) | FHS (µg) | PEG-FA (µg) | DNA (µg) | siRNA-FAM (µg) | Particle size (nm) | Zeta potential (mV) | FSM/ FHS/ PEG-FA/DNA mass ratio (w/w/w/w %) |
|---|---|---|---|---|---|---|---|---|
PEI/DNA (N/P = 5) | – | – | – | 5 | – | 309.31 ± 17 | + 6.4 ± 2.9 | N/P = 5 |
FSM 1/DNA | 100 | – | – | 5 | – | 264.54 ± 26 | −8.2 ± 3.1 | 20:0:0:1 |
FSM 2/DNA | 500 | – | – | 5 | – | 279.71 ± 21 | −5.4 ± 1.2 | 100:0:0:1 |
FSM 3/DNA | 1000 | – | – | 5 | – | 292.61 ± 35 | + 2.9 ± 0.55 | 200:0:0:1 |
FHS 1/DNA | – | 100 | – | 5 | – | 302.25 ± 24 | + 9.1 ± 1.4 | 0:20:0:1 |
FHS 2/DNA | – | 500 | – | 5 | – | 318.71 ± 09 | + 5.5 ± 2.4 | 0:100:0:1 |
FHS 3/DNA | – | 1000 | – | 5 | – | 307.42 ± 22 | + 10.9 ± 5.1 | 0:200:0:1 |
FSMPF 1/DNA | 100 | – | 25 | 5 | – | 327.44 ± 21 | + 9.9 ± 2.3 | 20:0:5:1 |
FSMPF 2/DNA | 500 | – | 125 | 5 | – | 343.71 ± 18 | −11.3 ± 3.1 | 100:0:25:1 |
FSMPF 3/DNA | 1000 | – | 250 | 5 | – | 353.43 ± 13 | −7.4 ± 1.5 | 200:0:50:1 |
FHSPF 1/DNA | – | 100 | 25 | 5 | – | 361.65 ± 31 | + 4.1 ± 1.64 | 0:20:5:1 |
FHSPF 2/DNA | – | 500 | 125 | 5 | – | 346.38 ± 19 | + 3.8 ± 1.62 | 0:100:25:1 |
FHSPF 3/DNA | – | 1000 | 250 | 5 | – | 379.51 ± 25 | + 3.2 ± 4.87 | 0:200:50:1 |
PEI/siRNA-FAM (N/P = 5) | – | – | – | – | 5 | 162.36 ± 21 | + 7.1 ± 2.36 | N/P = 5 |
FSM 1/siRNA-FAM | 100 | – | – | – | 5 | 153.21 ± 49 | −4.4 ± 1.72 | 20:0:0:1 |
FSM 2/siRNA-FAM | 500 | – | – | – | 5 | 169.1 ± 31 | −2.1 ± 0.19 | 100:0:0:1 |
FSM 3/siRNA-FAM | 1000 | – | – | – | 5 | 160.75 ± 3 | + 5.2 ± 0.26 | 200:0:0:1 |
FHS 1/siRNA-FAM | – | 100 | – | – | 5 | 153.26 ± 14 | + 6.8 ± 2.35 | 0:20:0:1 |
FHS 2/siRNA-FAM | – | 500 | – | – | 5 | 162.42 ± 8 | + 4.6 ± 0.96 | 0:100:0:1 |
FHS 3/siRNA-FAM | – | 1000 | – | – | 5 | 159.16 ± 2 | + 8.9 ± 3.89 | 0:200:0:1 |
FSMPF 1/siRNA-FAM | 100 | – | 25 | – | 5 | 162.81 ± 21 | + 4.4 ± 1.35 | 20:0:5:1 |
FSMPF2/siRNA-FAM | 500 | – | 125 | – | 5 | 158.52 ± 2 | −8.7 ± 3.44 | 100:0:25:1 |
FSMPF 3/siRNA-FAM | 1000 | – | 250 | – | 5 | 160.46 ± 3 | + 5.1 ± 1.28 | 200:0:50:1 |
FHSPF 1/siRNA-FAM | – | 100 | 25 | – | 5 | 157.54 ± 7 | −7.9 ± 0.54 | 0:20:5:1 |
FHSPF 2/siRNA-FAM | – | 500 | 125 | – | 5 | 163.07 ± 2 | + 3.9 ± 1.1 | 0:100:25:1 |
FHSPF 3/siRNA-FAM | – | 1000 | 250 | – | 5 | 154.75 ± 4 | + 7.4 ± 1.91 | 0:200:50:1 |
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