Fig. 2 | Scientific Reports

Fig. 2

From: Calcium modulates growth and biofilm formation of Lactobacillus acidophilus ATCC 4356 and Lactiplantibacillus plantarum ATCC 14917

Fig. 2

Calcium-dependent growth kinetics of L. acidophilus ATCC 4356 (a–f) and L. plantarum ATCC 14917 (g–l) subcultures in CDM supplemented with 1% Wolfe trace mineral solution. MRS (a,g), CDM (b,h), and mCDM (c,i) subculture growth curves as monitored by OD600. All error bars represent the standard deviation from three biological replicates with ≥ 2 technical replicates. Corresponding growth parameters for each condition: lag time (d,j), growth rate (e,k), and max OD (f,l) represent the mean ± SEM of three biological replicates with ≥ 2 technical replicates. All growth parameters were calculated using nonlinear regression curve fitting to a 4-parameter Logistic equation with GraphPad Prism 9 software. Significance was determined by one-way ANOVA with Tukey multiple comparison test: ns (not significant), *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001.

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