Fig. 3 | Scientific Reports

Fig. 3

From: Calcium modulates growth and biofilm formation of Lactobacillus acidophilus ATCC 4356 and Lactiplantibacillus plantarum ATCC 14917

Fig. 3

Effect of calcium on total biofilm formation for L. acidophilus ATCC 4356 (a-b) and L. plantarum ATCC 14917 (c-d). Biofilms formed by MRS, CDM, and mCDM subcultures of each species grown in CDM supplemented with Wolfe trace minerals solution were quantified by crystal violet (CV) staining as measured by UV-visible absorbance at 595 nm. All error bars represent the standard deviation of three biological replicates, each with ≥ 2 technical replicates. Significance was determined by one-way ANOVA with Tukey multiple comparison test: ns (not significant), *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001.

Back to article page