Fig. 4
Effect of chelator on Ca2+-dependent total biofilm formation and growth kinetics of L. acidophilus ATCC 4356 (a,c) and L. plantarum ATCC 14917 (b,d). Total biofilm levels (a,b) were determined by crystal violet staining (CV assay) as measured by A595 after 48 h. Calcium only effects from (a) and (b) were presented in Fig. 3b and d. Growth kinetics (c,d) were measured by monitoring the OD600. Measured data are shown with error bars as SD of three biological replicates, each with ≥ 2 technical replicates. Growth parameters for each condition: lag time (e), growth rate (f), and maxOD (g) represent the mean ± SEM of three biological replicates with ≥ 2 technical replicates. The growth parameters were calculated using nonlinear regression curve fitting to a 4-parameter Logistic equation with GraphPad Prism 9 software. Significance was determined by one-way ANOVA with Tukey multiple comparison test: ns (not significant), *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001.
