Fig. 7
Total biofilm formation (a) of L. acidophilus ATCC 4356 grown with varied calcium concentrations in CDM medium supplemented with 1% Wolfe trace mineral solution and 50 mM EGTA. Total biofilm was quantified by crystal violet staining (CV assay) as measured by A595. Data used for fitting are technical replicates from three biological replicates. Error bars are shown as SD of these four representative replicates. Data in (a) was fitted by DynaFit with a 1:1 binding model (b) to give Kd = 46 ± 0.7 µM. The fitting script is shown in Supplementary Fig. S12.
