Fig. 1
From: TNF-α inhibits Epstein Barr virus reactivation through the GPX4 mediated glutathione pathway
TNF-α inhibits EBV reactivation in vitro. (A) Raji cells were treated with TPA (20 ng/ml) and different concentrations of TNF-α for 24 h. Western blotting was performed with the indicated antibodies. (B) EBV DNA was extracted from Raji cells treated with TPA (20 ng/ml) or different concentrations of TNF-α, and the EBV DNA concentration was measured by digital PCR. (C) RNA was extracted from Raji cells treated with TPA (20 ng/ml) or TNF-α (200 ng/ml), and the mRNA expression levels of the EBV genes BRLF1 and BZLF1 were measured by real-time PCR. (D) LCL cells were treated with TPA (20 ng/ml) and different concentrations of TNF-α for 24 h. Western blotting was performed with the indicated antibodies. (E) EBV DNA was extracted from LCL cells treated with TPA (20 ng/ml) or TNF-α (200 ng/ml), and the EBV DNA concentration was measured by digital PCR. (F) Raji cells were induced with TPA (20 ng/ml) and TNF-α (200 ng/ml) for 24 h and harvested for immunofluorescence assays with Rta (red) and Zta (green) antibodies. The data shown are representative of three independent experiments. The data are presented as the means ± SDs. *P < 0.05; **p < 0.01 by one-way ANOVA.
