Fig. 4

TNF-α inhibits the EBV reactivation state by affecting latent ferroptosis. (A) Mitochondrial morphology of Raji cells treated with TPA (20 ng/ml) or TNF-α (200 ng/ml) for 24 h was observed using transmission electron microscopy. (B) MDA contents of Raji-shRNA-control and Raji-shRNA-hGPX4-2 cells treated with TPA (20 ng/ml) or TNF-α (200 ng/ml) for 24 h. (C) GSH/GSSG ratios of Raji-shRNA-control and Raji-shRNA-hGPX4-2 cells treated with TPA (20 ng/ml) or TNF-α (200 ng/ml) for 24 h. (D) Intracellular Fe2 + levels were detected in a FerroOrange Assay of Raji-shRNA-control and Raji-shRNA-hGPX4-2 cells, which were treated with TPA (20 ng/ml) and TNF-α (200 ng/ml) for 24 h. GFP indicates the effect of the lentivirus. (E) Lipid ROS levels were analysed using a Liperfluo fluorescence probe in Raji-shRNA-control and Raji-shRNA-hGPX4-2 cells, which were treated with TPA (20 ng/ml) and TNF-α (200 ng/ml) for 24 h. GFP indicates the effect of the lentivirus. The data shown are representative of three independent experiments. The data are presented as the means ± SDs. *P < 0.05; **p < 0.01 by one-way ANOVA.