Fig. 2 | Scientific Reports

Fig. 2

From: Investigating hepatic fibrosis heterogeneity by three-dimensional imaging in metabolic dysfunction-associated steatotic liver disease

Fig. 2

Collagen type I staining in cleared liver tissue. 3D images represent a part of the whole liver tissue stained with Coll-1 antibody. Digital pathological assessment shows more pronounced fibrillar staining as the disease progresses (A) F0, (B) F1, (C) F2, (D) F3, and (E) F4 in MASLD. Coll-1 antibody specificity is compared with its corresponding 2D histological liver sections (right). (Coll-1: Collagen type I; Green, nuclei; Blue, scale bar: 200 μm, left-bottom images are captured with a 40X magnification). 3D images were created using Leica Application Suite X (Version 3.5.5.19976, URL: https://www.leica-microsystems.com/products/microscope-software/p/leica-las-x-ls/).

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