Fig. 3 | Scientific Reports

Fig. 3

From: Investigating hepatic fibrosis heterogeneity by three-dimensional imaging in metabolic dysfunction-associated steatotic liver disease

Fig. 3

Elastin staining in cleared liver tissue. 3D representative images from a part of the whole liver tissue (left) show intense Elastin staining in fibrotic areas as the disease progresses (A) F0, (B) F1, (C) F2, (D) F3, and (E) F4 and histological assessment is compared with its corresponding 2D Sirius Red staining (right). (Elastin: Yellow, Nuclei: Blue, scale bar: 200 μm, left-bottom images are captured with a 40X magnification). 3D images were created using Leica Application Suite X (Version 3.5.5.19976, URL: https://www.leica-microsystems.com/products/microscope-software/p/leica-las-x-ls/).

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