Fig. 4 | Scientific Reports

Fig. 4

From: Investigating hepatic fibrosis heterogeneity by three-dimensional imaging in metabolic dysfunction-associated steatotic liver disease

Fig. 4

Imaging of CLARITY-processed intact liver biopsy reveals extensive fibrosis heterogeneity between optical sections in MASLD. (A) Three different optical sections obtained from an intact liver biopsy sample are evaluated and assessed with a different fibrosis stage by an expert pathologist. Whole liver biopsies from different stages of liver fibrosis (B) F0, (C) F1, (D) F2, (E) F3 and (F) F4 are stained with Collagen type I without histological sectioning. (B–F) Histogram analysis shows the average distribution in optical sections per whole biopsy sample using CPA approach. (G) 3D histogram analysis shows the percentage of optical sections per whole biopsy sample in fibrosis groups and emphasizes the overlap between F3 and F4 stage. (CPA: Collagen proportionate area). 3D images were created using Imaris Viewer (Version 10.1.0, URL: https://imaris.oxinst.com/imaris-viewer).

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