Fig. 1
AR/Ar mRNA levels are downregulated in the brains of male patients with early AD pathology and isolated microglia from male AppNL−G−F/NL−G−F mice. (a) Schematic of human brain sample selection based on Braak staging as determined according to senile plaque (SP) levels (0–C) and neurofibrillary tangle (NFT) levels (0–VI). non-AD (control): SP = 0–A, NFT = 0–II; the patients with early AD pathology (mild AD): SP = C, NFT = III–IV. Total RNA was extracted from the precunei of control and mild AD samples for quantitative PCR (qPCR). (b, c) AR expression levels in the human precuneus determined via qPCR. Relative expression levels are plotted as means ± standard errors of means (SEMs). Data are shown for males (b) and females (c) [Control (n = 8) and mild AD (n = 8) in males; control (n = 6) and mild AD (n = 3) in females]. (d) Schematic of total RNA preparation from mouse cerebral cortices and microglia isolated via magnetic-activated cell sorting (MACS). Microglia were isolated from one-half of the cerebral cortices of 8-month-old male wild-type (WT) and AppNL−G−F/NL−G−F (App) mice via MACS. Total RNA was extracted from the remaining cerebral cortices and isolated microglia. (e, f) Relative Ar expression levels in mouse cerebral cortex (e) and isolated microglia (f) measured using qPCR. Data are presented as means ± SEMs [WT (n = 3) and App (n = 3)]. *p < 0.05 and **p < 0.01 by Student’s t-test (b, c, e and f).
