Fig. 3
Long-term castration does not affect Aβ pathology or dystrophic neurites in AppNL−G−F/NL−G−F mice. (a–c) Representative immunofluorescence images showing IBA1 (green) and Aβ (red) expression, along with merged images, in the cerebral cortex (a), hippocampus (b), and amygdala (c) of 18-month-old male Sham- or Cast- WT and App mice. Scale bars: 100 μm. (d, e) Quantification of immunofluorescence images from (a–c). Percentage of immunopositive areas for Aβ (d) and IBA1 (e) in the cerebral cortex (Cx), hippocampus (Hip), and amygdala (Amy). f Representative immunofluorescence images showing BACE1 (green) and Aβ (red) expression, along with merged images, in the cerebral cortex of 18-month-old male Sham- or Cast- App mice. Scale bar: 100 μm. (g) Quantification of immunofluorescence images from (f). Percentage of BACE1 immunopositive areas (f). Data are presented as means ± SEMs (d, e and g) [Sham-WT mice (n = 6), Cast-WT mice (n = 7), Sham-App mice (n = 3), and Cast-App mice (n = 7–8); three sections quantified per mouse]. *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001 by two-way ANOVA with Bonferroni correction.
