Fig. 4
Long-term systemic androgen deprivation does not affect cognitive function in AppNL−G−F/NL−G−F mice. (a) Experimental protocol for the Barnes maze test using 18-month-old male Sham- or Cast- WT and App mice [Sham-WT mice (n = 13), Cast-WT mice (n = 15), Sham-App mice (n = 6), and Cast-App mice (n = 14)]. (b) Latency to enter the target hole during the training session. Each plot represents the mean ± SEM of four trials per group. Two-way repeated measures ANOVA with Bonferroni correction determined differences (*p < 0.05: between groups on each trial day, ####p < 0.0001: vs. Day1 in all groups). (c–f) Spatial memory was assessed during the probe test period based on the percentage of time spent in quadrant area (c), latency to first approach to the target hole (d), the number of primary errors (e), and the percentage of total errors (f). Data are presented as means ± SEMs. *p < 0.05 by two-way ANOVA with Bonferroni correction. (g) Latency to enter the target hole during the reversal test period. Each graph shows the means ± SEMs of the latency over four trials per group. Two-way ANOVA with Bonferroni correction determined differences.
