Table 1 Primer, probe, and internal control (IC) sequences.

From: Accurate quantification of cell-free Ceruloplasmin mRNA as a biomarker for early detection of hepatocellular carcinoma

Amplification round

Primers/probe

Sequence (5′-3′)

1st PCR round

Forward primer

(CP-Fo)

CGACGTAAAACGACGGCCAGT-CCATCTGAAAGCCGGTTTGC

Reverse primer

(CP-Ro)

CTGGCATATCATGACATACGACCTGA-ACATGCTTCCCACGG

Forward primer

(IC-Fo)

CGACGTAAAACGACGGCCAGT-ACTAGCGTGCCTTTGTAA

Reverse primer

(IC-Ro)

CTGGCATATCATGACATACGACCTGA-GAGCGATACGAGCA

2nd PCR round

Forward primer

(CP-Fi)

CCAGGTCCAGGAGTGTAA

Forward primer

(IC-Fi)

CTCATTCGTTTCGGAAGAGC

Reverse primer

(U-Ri)

CTGGCATATCATGACATACGACCTGA

Internal control’s sequence (IC)

CGGCGTAATACGACTCACTATAGGGATGAACCGACGACGACTAC

AGCGTGCCTTTGTAAGCACAAGCTGATGAGTACGAACTTATGTAC

TCATTCGTTTCGGAAGAGCGGGCGGCTGCTCGCGGATACCCGTAC

CTCGGGTTTCCGTCTTGCTCGTATCGCTCGAGAACGCAAGTTCTGT

TAACGTGAGTCTTGTAAAACCTTCTTTTTACGTTTACTCTCGTGTTA

AAAATCTGAATTCTTCTAGAGTTCCTGATCTTC

  1. Note: The underlined regions indicate synthetic non-human sequences added to the 5′ ends.
Back to article page