Fig. 9
CHIT1 + neutrophils was the terminally differentiated myeloid cells subpopulation and could interact with other immune cells via ICAM1-(ITGAM + ITGB2) pathway. (A) Pseudotime analysis of myeloid cells trajectory. This panel showed a pseudotime analysis of cell trajectories using a 2D plot with two principal components component 1 and component 2. The color gradient represented pseudotime, ranging from early blue to later edstages of cell development or differentiation. The plot illustrates the progression of cells along the trajectory, with branches indicating potential bifurcation points. (B) Cell type distribution along pseudotime. This panel displayed the distribution of different cell types along the pseudotime trajectory. The plot used a 2D representation with component 1 and component 2. Each color represented a different cell type. The plot showed how the proportion of each cell type changes over pseudotime, indicating potential transitions between cell states. (C) Condition-specific pseudotime trajectory. This panel illustrated the pseudotime trajectory for different conditions. The plot used a 2D representation with component 1 and component 2. The color coding helped visualize how cells from different conditions followed similar or distinct trajectories over pseudotime. (D) Cell-chat analysis showed CHIT1 + neutrophils could interact with other immune cell types, including B cells, T cells, NK cells, platelets, erythroid cells, D14 + + monocytes/macrophages, dendritic cells (DC) and CD14 + monocytes/macrophages. (E) Dot plot of ICAM1-(ITGAM + ITGB2) pathway activity in interaction of CHIT1 + neutrophils with other immune cells. F: Violin plots showing the expression of ICAM1, ITGAM, and ITGAX genes across different immune cell types.
