Fig. 3
From: OTX2 expression contributes progression of gastric cancer in young adults
The efects of OTX2 expressionon on biologic processes in NUGC-4 cells after downregulated OTX2 expression. (A–C) The morphology of some NUGC-4 cells changed from round to spindle cells after interference with OTX2 expression; (D–F, T) Interference of NUGC-4 expression attenuated colony formation in NUGC-4 cells. (*P < 0.05); (S) Compared with SiRNA1 and siRNA3, siRNA2 had the best effect on interfering OTX2 expression. The upper bands represent OTX2 expression levels. The bands below represent β-actin expression levels. (G–I, V) Knocking-down expression of OTX2 increased the rate of apoptosis of NUGC-4 cells (*P < 0.05); (J–L, U) Compared with cells transfected with control siRNA (NC-siRNA), interference of OTX2 expression markedly inhibited the invasion of NUGC-4 cells; (M–R) Interference of OTX2 expression by siRNA inhibited migration of NUGC-4 cells.
