Fig. 3

Size versus age for fish used for vital fluorescent staining in all three temperature treatments (refer to Fig. 1D). Rate of growth (best-fit linear regression, p < 0.05): 0.24 mm SL⋅day⁻¹ at + 4 °C (red, R² = 0.88, n = 44), 0.25 mm SL⋅day⁻¹ at + 2 °C (yellow, R² = 0.96, n = 47), and 0.26 mm SL⋅day⁻¹ at + 0 °C ([ambient], blue, R² = 0.93, n = 43). Fish size was significantly larger at + 4 °C than at + 2 °C and + 0 °C (ambient), and fish reared at + 2 °C were significantly larger than those reared at + 0 °C (ambient) (ANCOVA with Tukey Contrasts, p < 0.05). SL = standard length, dpf = days post-fertilization.