Fig. 5 | Scientific Reports

Fig. 5

From: Salmonid sensory system development is affected by climate change driven temperature increases

Fig. 5

Size of canal neuromasts (CNs; in SO-f and PM-d canal series) and superficial neuromasts (SNs; in adl series) versus fish size (mm standard length [SL], left) and age (days post-fertilization [dpf], right) derived from SEM images. Neuromast size (length) was compared among treatments using ANCOVA with Tukey contrasts, α = 0.05; refer to text and S3, S4 for details. (A) Size of canal neuromasts in the SO-f canal (n = 70 neuromasts) vs. fish size was significantly larger in fish reared at higher temperatures (red = + 4 °C, p = 0.002; yellow = + 2 °C, p < 0.001) than at ambient temperature (+ 0 °C [ambient], blue). (B) Size of CNs PM-d canal (n = 84 neuromasts) was not significantly different among temperature treatments (ANCOVA, p > 0.05, S4). Data presented in A and B include only SO-f and PM-d canal neuromasts prior to their enclosure in canals, after which (in larger/older fishes) they could not be visualized with SEM. (C) Size of adl SNs (n = 185 neuromasts) was not significantly different among temperature treatments (ANCOVA, p > 0.05, S4). SO-f: supraorbital canal segments associated with frontal bone, PM-d: preopercular-mandibular canal segments associated with the dentary bone, adl = antero-dorsal SN line; refer to Fig. 1A.

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