Table 2 Antibiotic resistance of used bacterial strain and vector.
From: A beginners guide to Sf9 and Sf21 insect cell line culture and troubleshooting
Bacteria strain/vectors | Feature | Antibiotic resistance (end concentration) |
|---|---|---|
DH10 MultiBac strain | Bacterial strain | Ampicillin (100 µg/µL) Kanamycin (50 µg/µL) Tetracycline (12.5 µg/µL) |
pUCDM-mCherry | Donor vector | Chloramphenicol (25 µg/µL) |
pFBDM-CSTF | Acceptor vector | Ampicillin (100 µg/µL) Gentamicin (50 µg/µL) |