Table 4 Real-time PCR thermal cycling conditions for the 35 S promoter, plant gene, and lectin.
From: Heat processing compromises GMO detection in soybean-enriched biscuits
Program: Preincubation (Cycle 1) | ||||
|---|---|---|---|---|
Mode: None | ||||
Segment | Target Temp (°C) | Time (s) | Ramp Rate (°C/s) | Acquisition |
1 | 95 | 900 | 20 | None |
Program: Amplification (Cycle45) | ||||
|---|---|---|---|---|
Mode: Quantification | ||||
Segment | Target Temp (°C) | Time (s) | Ramp Rate (°C/s) | Acquisition |
1 | 95 | 0 | 20 | None |
2 | 60 | 25 | 20 | Single |
3 | 72 | 15 | 20 | None |
Program: Cooling (Cycle 1) | ||||
|---|---|---|---|---|
Mode: None | ||||
Segment | Target Temp (°C) | Time (s) | Ramp Rate (°C/s) | Acquisition |
1 | 40 | 30 | 20 | None |
