Table 5 Primers and probes used in the detection of plant genes, the lectin gene, and the 35 S promoter.
From: Heat processing compromises GMO detection in soybean-enriched biscuits
Gene | Primer/Probe | Sequence (5’–3’) | Length (bp) |
|---|---|---|---|
Plant gene | Forward | CGAAATGGTAGACGCTACG | 19 |
Reverse | CCATTGAGTCTCTGCACCTATC | 22 | |
Probe | Fam-TCCAAATTCAGAGAAACCCT-Tamra | 24 | |
Lectin gene | Forward (Le1_F) | CCAGCTTCGCCGCTTCCTTC | 20 |
Reverse (Le1_R) | GAAGGCAAGCCCATCTGCAAGCC | 23 | |
Probe (Le1_Prob) | 5’-Fam-CTTCACCTTCTATGCCCCTGACAC-Tamra-3’ | 24 | |
35 S promoter | Forward | GCTCCTACAAATGCCATCA | 19 |
Reverse | CTCCAAATGAAATGAACTTCC | 21 | |
Probe | 5’-Fam-CAAAGATGGACCCCCACCCACG-Tamra-3’ | 22 | |
RR-specific | RuptarF | TTCATTCAAAATAAGATCATACATACAGGTT | 31 |
RuptarR | GGCATTTGTAGGAGCCACCTT | 21 | |
RupTPrb | 5’-6FAM-C + CT + TT + TC + CA + TT + TGGG–TMR | 15 |
