Table 6 Representive DNA concentration and purity (A260/A280) of biscuit samples extracted using Roche high pure DNA Kit.
From: Heat processing compromises GMO detection in soybean-enriched biscuits
RRSFa addition (%) | Cooking Temperature (°C) | A260/A280 | DNA (ng/µL) |
|---|---|---|---|
0 | Dough | 0.617 | 10.866 |
0.1 | Dough | 0.864 | 11.234 |
0.5 | Dough | 0.988 | 10.564 |
0.9 | Dough | 1.132 | 10.267 |
2.0 | Dough | 1.056 | 13.521 |
50 | Dough | 1.834 | 14.679 |
100 | Dough | 1.967 | 11.347 |
0 | 190 | 1.698 | 13.024 |
0.1 | 190 | 1.875 | 14.505 |
0.5 | 190 | 1.467 | 8.306 |
0.9 | 190 | 1.651 | 11.048 |
2.0 | 190 | 1.551 | 14.363 |
50 | 190 | 1.787 | 10.376 |
100 | 190 | 1.821 | 10.421 |
0 | 200 | 1.731 | 13.204 |
0.1 | 200 | 1.834 | 6.033 |
0.5 | 200 | 1.952 | 11.532 |
0.9 | 200 | 1.897 | 13.306 |
2.0 | 200 | 1.902 | 9.449 |
50 | 200 | 1.888 | 15.31 |
100 | 200 | 1.703 | 10.003 |
0 | 210 | 1.578 | 15.664 |
0.1 | 210 | 1.421 | 10.948 |
0.5 | 210 | 1.882 | 12.645 |
0.9 | 210 | 1.718 | 18.029 |
2.0 | 210 | 1.397 | 13.996 |
50 | 210 | 1.864 | 10.974 |
100 | 210 | 1.994 | 17.254 |
