Table 7 Crossing point (CP) data of plant gene screening, lectin gene screening, 35 S screening regions performed in real-time PCR in biscuit samples.
From: Heat processing compromises GMO detection in soybean-enriched biscuits
RRSFa addition (%) | Cooking Temperature (°C) | CP values for 35 S promotor region | Fit Point for 35 S promotor region | Lcctin Gene | Plant Gene |
|---|---|---|---|---|---|
0 | 190 | 39.22 | - | - | + |
0.1 | 190 | 32.26 | + | + | + |
0.5 | 190 | 28.54 | + | + | + |
0.9 | 190 | 21.57 | + | + | + |
2 | 190 | 19.33 | + | + | + |
50 | 190 | 17.01 | + | + | + |
100 | 190 | 15.16 | + | + | + |
0 | 200 | 23.91 | - | - | + |
0.1 | 200 | 25.62 | + | + | + |
0.5 | 200 | 25.01 | + | + | + |
0.9 | 200 | 25.32 | + | + | + |
2 | 200 | 16.19 | + | + | + |
50 | 200 | 16.05 | + | + | + |
100 | 200 | 16.41 | + | + | + |
0 | 210 | 24.30 | - | - | + |
0.1 | 210 | 24.62 | + | + | + |
0.5 | 210 | 24.91 | + | + | + |
0.9 | 210 | 25.69 | + | + | + |
2 | 210 | 16.98 | + | + | + |
50 | 210 | 18.92 | + | + | + |
100 | 210 | 16.01 | + | - | + |
0%RR Soya NCc | - | 39.01 | - | - | - |
0.1% RR Soya | - | 24.00 | + | + | + |
1%RR Soya PCb | - | 16.14 | + | + | + |