Table 7 Crossing point (CP) data of plant gene screening, lectin gene screening, 35 S screening regions performed in real-time PCR in biscuit samples.

From: Heat processing compromises GMO detection in soybean-enriched biscuits

RRSFa addition (%)

Cooking Temperature (°C)

CP values for 35 S promotor region

Fit Point for 35 S promotor region

Lcctin Gene

Plant Gene

0

190

39.22

-

-

+

0.1

190

32.26

+

+

+

0.5

190

28.54

+

+

+

0.9

190

21.57

+

+

+

2

190

19.33

+

+

+

50

190

17.01

+

+

+

100

190

15.16

+

+

+

0

200

23.91

-

-

+

0.1

200

25.62

+

+

+

0.5

200

25.01

+

+

+

0.9

200

25.32

+

+

+

2

200

16.19

+

+

+

50

200

16.05

+

+

+

100

200

16.41

+

+

+

0

210

24.30

-

-

+

0.1

210

24.62

+

+

+

0.5

210

24.91

+

+

+

0.9

210

25.69

+

+

+

2

210

16.98

+

+

+

50

210

18.92

+

+

+

100

210

16.01

+

-

+

0%RR Soya NCc

-

39.01

-

-

-

0.1% RR Soya

-

24.00

+

+

+

1%RR Soya PCb

-

16.14

+

+

+

  1. aRRSF: Roundup Ready Soy Flour.
  2. bPC: Positive Control.
  3. cNC: Negative Control.