Table 8 CRM-Normalized relative EPSPS signal and crossing point (CP) values for RR soybean and lectin gene detected in dough and biscuit Samples.

From: Heat processing compromises GMO detection in soybean-enriched biscuits

RRSF1 addition (%)

Cooking Temperature

CP values for RR Soybean Gene

CP values for Lectin Gene

CRM-Normalized Relative EPSPS Signal (%)

0

Dough

40 ± 0.002a

40 ± 0.00a

0.00 ± 0.00d

0.1

Dough

38.76 ± 0.71 a

28.42 ± 4.26bcd

0.10 ± 0.00d

0.5

Dough

37.77 ± 0.10 a

34.99 ± 0.45ab

0.48 ± 0.00d

0.9

Dough

30.86 ± 3.03b

27.51 ± 3.47cd

0.90 ± 0.00cd

2.0

Dough

37.55 ± 0.03 a

33.70 ± 0.49abc

2.21 ± 0.06c

50

Dough

30.32 ± 0.03b

26.25 ± 1.75d

49.85 ± 1.12b

100

Dough

28.39 ± 0.12b

19.27 ± 1.59e

102 ± 8.21a

0

190

40 ± 0.00a

40 ± 0.00a

0 ± 0.00 d

0.1

190

39.20 ± 0.79ab

27.86 ± 4.74def

0.11 ± 0.01 d

0.5

190

39.00 ± 0.95 ab

32.81 ± 0.37bc

0.33 ± 0.00 d

0.9

190

36.86 ± 1.29cd

30.92 ± 1.01bcd

0.61 ± 0.11 d

2.0

190

38.22 ± 0.47 abc

31.94 ± 1.15bcd

0.55 ± 0.24 d

50

190

33.53 ± 0.51e

28.33 ± 1.57de

42.49 ± 9.68b

100

190

28.01 ± 0.01g

21.27 ± 0.44g

64.53 ± 1.21a

0

200

40 ± 0.00a

40 ± 0.00a

0 ± 0.00 d

0.1

200

39.54 ± 0.01a

28.26 ± 0.25de

0.06 ± 0.01 d

0.5

200

37.32 ± 0.57bcd

32.74 ± 0.53bc

0.28 ± 0.01 d

0.9

200

36.03 ± 1.11d

29.78 ± 1.82bcd

0.30 ± 0.16 d

2.0

200

36.02 ± 0.80d

31.89 ± 1.91bcd

0.41 ± 0.38 d

50

200

31.22 ± 2.19f

20.58 ± 4g

28.71 ± 7.72c

100

200

28.24 ± 0.45g

24.03 ± 3.06fg

61.73 ± 5.54a

0

210

40 ± 0.00a

40 ± 0.00a

0 ± 0.00 d

0.1

210

39.59 ± 0.40a

28.84 ± 4.38cd

0.04 ± 0.03 d

0.5

210

35.62 ± 2.60d

32.90 ± 1.44bc

0.23 ± 0.07 d

0.9

210

35.78 ± 2.72d

28.51 ± 3.16cde

0.17 ± 0.07d

2.0

210

38.87 ± 1.12 abc

33.89 ± 0.86b

0.40 ± 0.27d

50

210

30.34 ± 0.31f

24.35 ± 4.18efg

22.69 ± 3.65c

100

210

29.74 ± 0.57fg

23.04 ± 0.82g

49.74 ± 1.30b

  1. 1RRSF: Roundup Ready Soy Flour.
  2. 2There is no statistically significant difference between means sharing the same letter within the same column (p < 0.05).