Fig. 2
From: Two paired HPV-59 cervical cancer cell lines with distinct chemoradioresistant phenotypes
AdMer35 and AdMer43 cells characterization. (A) AdMer35 and (B) AdMer43 cells karyotypes, GTG staining. Proliferation (C) and migration (D) activity of HeLa, SiHa, AdMer35 and AdMer43 cells monitoring using xCELLigence RTCA system. Data were recorded once per 30 min during 100 h and expressed as Cell index. (E, F) Expression of ki67 in human cervical cancer cells AdMer35 and AdMer43 was measured after cell staining with primary anti-Ki67 monoclonal antibody followed by staining with secondary polyclonal anti-hIgG- AF488 antibody (green channel). Microphotographs (E) were captured with a Zeiss LSM710 confocal microscope using a Plan-Apochromat 63×/1.40 Oil DIC M27 objective. Nuclei were stained with DAPI (blue channel). FASC analysis (F) was performed with NovoCyte 3000 flow cytometer, 10.000 events were acquired for each sample. The data were processed using NovoExpress software.
