Abstract
Chronic exposure to nicotine significantly exacerbates periodontitis, a prevalent inflammatory disease, by inducing cellular processes such as autophagy and inflammation in gingival fibroblasts. Current therapies often fail to fully address these cellular alterations in smokers, highlighting a need for innovative therapeutic and regenerative approaches. This study explores the therapeutic potential of Platelet-Rich Plasma (PRP), a blood-derived product, to modulate nicotine-induced biological activities in primary gingival fibroblasts, particularly in the case of periodontitis in smokers. Gingival fibroblasts were treated with increasing concentrations of nicotine, which led to senescence and autophagy. Nicotine at high concentrations triggered cellular vacuolization, and a decrease in metabolism, viability and proliferation. Concomitant cell treatment with 10% PRP reversed nicotine effects and significantly increased cell migration potential. In Caenorhabditis elegans, PRP reduced the nicotine-induced autophagic activity. A screening of the gingival fibroblast secretome revealed a modulation of autophagy-related cytokines in response to nicotine and/or PRP. The findings demonstrate that PRP could effectively inhibit nicotine-induced autophagy in gingival fibroblasts, offering insights into its possible use as a therapeutic tool for managing periodontitis in smokers. The study underscores the potential of PRP in altering disease progression by modulating key cellular processes affected by smoking.
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All data generated or analyzed during this study are included in this published article. Experiments were realized in accordance with ARRIVE guidelines.
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Acknowledgements
The C. elegans strains were kindly provided by the C. elegans Genetics Center (University of Minnesota, MN, USA). A great thanks to Nicolas Liaudet, who coded the script for picture quantification on QuPath and to the Bioimaging facility of the UNIGE for their help. We thank Vanessa Fétaud Lapierre and Gaël Vieille for allowing us the use of the UFA facility and its equipment.
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S.B. and C.G. designed the study. S.B, JA.C, performed the cell experiments. J.VS. designed and performed the experiments in *C. elegans* . A.T, M.C, S.V and C.G. contributed to the final version of the manuscript.
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The authors declare the following competing interest: S.B. is head project manager, cell therapy, at Regen Lab; an employee of ouis employed by and A.T. is the CEO of Regen Lab SA. The authors receive salary from Regen Lab SA. The study received financial support, materials and reagents from Regen Lab SA. Regen Lab SA develops and commercializes products in the field related to this study and could reasonably benefit from its findings. All other authors declare no competing interests.
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Vérièpe-Salerno, J., Cancela, J.A., Vischer, S. et al. Platelet-rich plasma promotes cellular recovery from nicotine-induced toxicity via autophagy modulation. Sci Rep (2026). https://doi.org/10.1038/s41598-026-38188-1
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DOI: https://doi.org/10.1038/s41598-026-38188-1


