Abstract
Accurately diagnosing mustard lung disease (MLD) presents a significant challenge due to its intricate nature and overlapping clinical features with other pulmonary conditions. A precise diagnosis is crucial for effective therapeutic management and optimizing patient care. Furthermore, understanding the metabolic shifts induced by sulfur mustard (SM) exposure is critical for elucidating the disease’s mechanisms and developing targeted interventions. This study employed an untargeted metabolomics and lipidomics profiling by liquid chromatography-mass spectrometry (LC-MS). We analyzed samples from MLD patients (n = 39; 20 mild, 19 moderate severity) and a control group (n = 14). We used multivariate/univariate statistical methods to identify distinguishing metabolites and lipids, and then performed pathway enrichment analysis to uncover the perturbed biochemical pathways. Our results demonstrated significant metabolic disruptions in MLD patients. We identified 16 metabolite panels capable of diagnosing mild MLD against controls, and 22 metabolite panels for moderate MLD versus controls (AUC > 0.85). Additionally, in comparison with the control group, four lipids were detected in the mild MLD group and five in the moderate MLD group (p-value < 0.05). Our findings reveal unique metabolite and lipid profiles and widespread disturbances across various metabolic pathways, including amino acid, butyrate, propanoate metabolism, and carnitine synthesis, which differentiate MLD from controls. This research represents the first investigation into metabolomic and lipidomic signatures that discriminate MLD from control groups using LC-MS. Significant metabolites show promise as candidate biomarkers for MLD diagnosis or prognosis and offer valuable insights for further research into the disease’s pathological mechanisms, pending validation in larger prospective cohorts.
Data availability
The raw data that support the findings of this study are available on permission request from the Director of Chemical Injuries Research Center, Hasan Bagheri as third party. The data are not publicly available due to restrictions e.g. their containing information that could compromise the privacy of research participants and third-party laws.
Abbreviations
- SM:
-
Sulfur mustard
- MLD:
-
Mustard lung disease
- COPD:
-
Chronic obstructive pulmonary disease
- NMR:
-
Nuclear magnetic resonance
- LC:
-
Liquid chromatography
- GC:
-
Gas chromatography
- MS:
-
Mass spectrometry
- HRCT:
-
High-resolution computed tomography
- PFT:
-
Pulmonary function tests
- HILIC:
-
Hydrophilic interaction liquid chromatography
- ACN:
-
Acetonitrile
- AF:
-
Ammonium formate
- FA:
-
Formic acid
- QC:
-
Quality control
- CV:
-
Coefficient of variability
- MSI:
-
Metabolomics standards initiative
- PCA:
-
Principal component analysis
- OPLS-DA:
-
Orthogonal projections to latent structures-discriminant analysis
- ROC:
-
Receiver-operating characteristic
- AUC:
-
Area under the ROC curve
- VIP:
-
Variable importance in projection
- MSEA:
-
Metabolite set enrichment analysis
- MDD:
-
Major depressive disorder
- VLCFA:
-
Very long-chain fatty acid
- EPA:
-
Eicosapentaenoic acid
- ARDS:
-
Acute respiratory distress syndrome
- ADMA:
-
Asymmetric dimethylarginine
- ROS:
-
Reactive oxygen species
- TCA:
-
Citric acid cycle
- HIF-1α:
-
Hypoxia-inducible factor 1-alpha
- NAC:
-
N-acetylcysteine
- PGE-1:
-
Prostaglandin E1
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Acknowledgements
The authors extend their gratitude to the study participants for their invaluable contributions to scientific advancement. Additionally, sincere appreciation is expressed to the Cohort Study of Iranian Chemical Injured (CICI Study) at Baqiyatallah University of Medical Sciences (Ethical approval: IR.BMSU.REC.1399.422) for their essential support in facilitating sample collection, preparation, and biobanking.
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This work was supported financially by the grant which has been taken from Baqiyatallah University of Medical Sciences.
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H.B.: Conceptualization, project administration, funding acquisition, writing—review and editing. U.K.: Methodology, formal analysis, visualization, writing—review and editing. M.G.: Conceptualization, supervision, project administration, writing—review and editing. B.F.N.M.Gh: Investigation, software, methodology, formal analysis, validation, writing—original draft, writing—review and editing.
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Nobakht M. Gh., B.F., Bagheri, H., Keshet, U. et al. A pilot study reveals plasma metabolomic and lipidomic signatures of mustard lung disease. Sci Rep (2026). https://doi.org/10.1038/s41598-026-39675-1
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DOI: https://doi.org/10.1038/s41598-026-39675-1