Fig. 2

Piezo1 in Runx2-expressing osteoblast-lineage cells partly mediates the effect of increased weight loading on body weight. (a–d) The effect of increased loading on body weight in mice with either intact Piezo1 expression (Piezo1^flox/flox) or reduced Piezo1 expression in Runx2-expressing osteoblast-lineage cells (Runx2-Cre;Piezo1^flox/flox). (a) Male Piezo1^flox/flox mice (control n = 15, load n = 13), (b) male Runx2-Cre;Piezo1^flox/flox mice (control n = 9, load n = 9), (c) female Piezo1 ^flox/flox + Runx2-Cre; Piezo1^flox/wt mice (control n = 19, load n = 17), and (d) female Runx2-Cre;Piezo1^flox/flox mice (control n = 5, load n = 8). (e) The effect of increased loading on retroperitoneal white adipose tissue (WAT) weight in male Piezo1^flox/flox (control n = 15, load n = 13) and Runx2-Cre;Piezo1^flox/flox (control n = 9, load n = 9) mice. Data are shown as mean ± SEM. Statistical analyses were performed using repeated measures ANOVA (for weight curves) or Student’s t-test (for retroperitoneal WAT). p < 0.05 was considered significant., n.s = non-significant.