Extended Data Fig. 1: Immediate early gene expression in the mPFC after CNO (1 mg/kg) or VEH administration via IP injection or MDA in the absence of the modified human muscarinic M3 G-protein-coupled receptor (DREADD).

The scatter bar blots represent mRNA levels of cFos, Arc and Zif268 in the mPFC of adult (12-week-old) C57Bl6/N mice 60 min after treatment with VEH or CNO given via MDA or IP injections; n(VEH/IP) = 7, n(CNO/IP) = 7, n(VEH/MDA) = 7, n(CNO/MDA) = 7. Each dot in the scatter bar plot represents an individual animal, and error bars represent s.e.m.