Fig. 2: Luciferase activity in tissues and their extracts.
a,b, Relative luminescence units (RLU) normalized to mg of protein tissue extracts of CAG–oFluc (a) and CAG–Venus/Akaluc (b) mice supplemented with their substrates (1 mM each, d-luciferin and AkaLumine-HCl, respectively). For comparison, tissue extracts were collected before the CAG–Cre cross (that is, CAG–LSL–oFluc and CAG–LSL–Venus/Akaluc) and from C57BL/6 mice, and are included in the graphs. From left to right, tissues exhibiting stronger signals were plotted in order. c, RLU of the tissue extracts of oFluc supplemented with 1 mM CycLuc1. d, Relative fluorescence units for the Venus reporter in CAG–Venus/Akaluc. n = 3 mice per group for tissue extracts. ∗P < 0.05; #P = 0.057; and ##P = 0.059 compared with C57BL/6 mice (Student’s t-test). e,f, Ex vivo imaging of tissues dissected from CAG–oFluc (e) and CAG–Venus/Akaluc (f) mice incubated with their substrates (1 mM). As negative controls, tissues from C57BL/6 mice were imaged under the same conditions (right). Scale, photons/s/cm2/sr. g, Ex vivo imaging of Venus fluorescence in tissues from CAG–Venus/Akaluc mice. The images were captured using VISQUE InVivo Smart-LF. Note that C57BL/6 mice exhibited autofluorescence in some tissues when imaged under the same conditions. Scale, intensity (0–65,025).
