Fig. 5: In vivo BLI of Cre-dependent reporter mice before the Cre cross. | Lab Animal

Fig. 5: In vivo BLI of Cre-dependent reporter mice before the Cre cross.

From: Development of two mouse strains conditionally expressing bright luciferases with distinct emission spectra as new tools for in vivo imaging

Fig. 5

a,b, Basal luminescence of CAG–LSL–oFluc (a) and CAG–LSL–Venus/Akaluc (b) mice at 10 min (dorsal side) and 50 min (ventral side) after IP injection of their substrate (100 mM d-luciferin, 5 µl per g.b.w. for oFluc; 15 mM AkaLumine-HCl, 5 µl per g.b.w. for Akaluc). Images acquired using four different exposure times are shown, and each panel includes three mice (from the top, two experimental mice and a C57BL/6 mouse, as a negative control; mouse sex: female, male and male, respectively). The hair on the dorsal and ventral sides of the mice was shaved. c, Basal luminescence of CAG-LSL-oFluc mice after IP injection of CycLuc1 (5 mM, 10 µl per g.b.w.). Images were acquired using the same mice as shown in a. Scale, photons/s/cm2. d,e, Ex vivo imaging of dissected brains incubated with d-luciferin (1 mM) (d) or AkaLumine-HCl (1 mM) (e). For longer exposure, the dissected brains of CAG–oFluc or CAG–Venus/Akaluc mice were removed for BLI because their bright luminescence also lit up other tissues, resulting in false-positive signals. f, Quantification of head luminescence for in vivo BLI. The black-filled boxes correspond to luminescence in C57BL/6 mice. n = 4 for reporter mice and n = 3 for C57BL/6 mice. ∗P < 0.05 and #P = 0.054 compared with C57BL/6 mice (Student’s t-test). g, Venus fluorescence imaging using VISQUE InVivo Smart-LF of dissected brains of CAG–Venus/Akaluc, CAG–LSL–Venus/Akaluc and C57BL/6 mice. Scale, intensity (0–65,025).

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