Fig. 1: AAV-EP: A robust pipeline to efficiently generate genetically modified mice. | Lab Animal

Fig. 1: AAV-EP: A robust pipeline to efficiently generate genetically modified mice.

From: Streamlining mouse genome editing by integrating AAV repair template delivery and CRISPR-Cas electroporation

Fig. 1

Starting with the production of zygotes by natural mating (or alternatively, in vitro fertilisation), the pipeline continues by introducing AAV-repair templates into embryo incubation drops. Next, CRISPR-Cas reagents (including Cas9 protein and sgRNAs) are electroporated into batches of up to 100 embryos, depending on the slide or cuvette size, followed by overnight culture until reaching the 2-cell stage. The surviving embryos are then surgically implanted into foster mice, and the resulting litters are screened for accurate targeting. Potential founders are identified and further bred to establish the new mouse colony.

Back to article page