Fig. 1: Metabolic parameters.
Evaluation of the metabolic profile of mice with early-life UNX and consuming a modified WD with high sugar, fat and salt for 12 and 20 weeks. a, Body weight curve in grams. b, Fat mass quantified by dual-energy X-ray absorptiometry scan. c, Serum insulin levels in a fed state in the first hours of the day. d, Intraperitoneal glucose tolerance test (ipGTT); *, compared with sham group; #, compared with UNX + WD 12 weeks group. e, Glucose uptake in isolated skeletal muscle (extensor digitorum longus) with (+) and without (−) insulin. f, The insulin-mediated glucose uptake presented as the change in glucose uptake with and without insulin in muscles from the same animal. 2DG, 2-deoxyglucose; prot, protein. The data are expressed as mean ± s.d., and the number of samples per method is described in Supplementary Table 2. For all parameters, one-way ANOVA was performed followed by Tukey’s multiple comparison test, except for the glucose tolerance test (d) where the two-way ANOVA test was performed. n.s., not significant, *P < 0.05, **P < 0.01, ***P < 0.001. #, Compared with the UNX + WD 12 weeks group.
