Table 3 Renal parameters in mice submitted to early age UNX and fed with high-salt WD for 12 and 20 weeks, compared with the sham-operated mice on RD

From: A novel model of cardiovascular–kidney–metabolic syndrome combining unilateral nephrectomy and high-salt–sugar–fat diet in mice

Renal parameters

Sham + RD

UNX + WD 12 weeks

UNX + WD 20 weeks

GFR:BW ratio (μl/min/g)

6.84 ± 3.06

3.09 ± 0.83*

3.94 ± 1.17*

Kidney complex II activity (pmol O2 µg−1 s−1)

6.05 ± 0.37

5.23 ± 0.94*

6.09 ± 0.62

Kidney complex IV activity (pmol O2 µg−1 s−1)

16.32 ± 0.72

14.05 ± 1.11*

16.64 ± 0.98#

Tubular injury score (0–10)

1.13 ± 0.94

6.53 ± 1.45*

8.00 ± 0.75*

Glomerular injury (%)

16.88 ± 3.53

45.05 ± 3.70*

58.72 ± 8.20*#

Glomerular area (µm2)

2,923 ± 766.1

4,425 ± 1,086*

6,414 ± 1,479*#

Fibrosis area (%)

3.08 ± 0.73

4.12 ± 1.68

6.05 ± 0.77*#

  1. Values are expressed as mean ± s.d. BW, body weight. Sham + RD (n = 12); UNX + WD 12 weeks (n = 12); UNX + WD 20 weeks (n = 8). For all parameters, one-way ANOVA was performed followed by Tukey’s multiple comparison test. *P < 0.05 compared with the corresponding sham group. #, P < 0.05 compared with the UNX + WD 12 weeks group.
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