Fig. 1: Comparison of the two mouse lines for ANL cell type-specific protein labeling.
From: Cell type-specific in vivo proteomes with a multicopy mutant methionyl tRNA synthetase mouse line
a, Insertion of the MetRS* cassette in the ROSA26 locus of wild-type mice (WT). b, Scheme of the cassette introduced in the first generation of the floxed-STOP-MetRS* mouse line. The right part shows mice expressing one (1xMetRS*, heterozygous animals) or two (2xMetRS*, homozygous animals) copies of the transgene after recombination by Cre. c, Scheme of the cassette introduced in the second-generation floxed MetRS*, showing all the introduced genes separated by 2A peptides, and the mRNA stabilization element (WPRE) at the end of the cassette. The right part shows mice expressing three (3xMetRS*, heterozygous animals) or six (6xMetRS*, homozygous animals) copies of the gene after recombination by Cre. SA, splice acceptor.
