Fig. 3: Methionine competition with ANL.
From: Cell type-specific in vivo proteomes with a multicopy mutant methionyl tRNA synthetase mouse line
a,c, FUNCAT in primary neurons from cortices of Nex-Cre::1xMetRS* mice (a) or Nex-Cre::3xMetRS* mice (c) labeled with ANL for 2 h in the presence (met (+)) or absence (met (−)) of methionine in the culture media. Scale bar, 100 µM. b,d, Quantification of the experiments shown in a (b) and c (d), including a 1-h time point and a no-ANL control. Multiple-comparison ANOVA was used to assess statistical significance, *P = 0.0437, ***P = 0.0001. Each dot represents the average of single-cell quantifications for one dish in at least three independent experiments with a minimum of two dishes per experiment. Number of neurons quantified are Nex-Cre::1xMetRS*, 1 h +met (2,085), 1 h −met (1,496), 2 h +met (1,904), 2 h −met (1,651); no ANL +met (1,189), no ANL −met (1,236); Nex-Cre::3xMetRS*, 1 h +met (814), 1 h −met (637), 2 h +met (558), 2 h −met (595), no ANL +met (520), no ANL −met (460). The bars and error bars represent the mean ± s.d. in all panels.
