Fig. 1: Effect of the anesthetic protocols on mouse vital parameters.

a, A schematic representation of the three anesthetic protocols. Surgery included the preparation of the pLN for intravital microscopy. Preop., preoperative. b,c, Induction time, defined as the time passing from drug administration to loss of righting reflex (b), and nonsurgical tolerance, defined as the time passing from loss of righting reflex to loss of pedal reflex (c). d, Oxygen saturation over time. The arrow indicates the time of BPP administration in all the three groups. e, Mortality rate for each group indicated as the percentage of mice unable to recover from anesthesia over the total number of mice anesthetized. f, Recovery time, defined as the time passing from the gain of pedal reflex to the recovery of the entire walking capacity. In b, c and f, lines and points indicate mean and individual values, respectively. In d and e, n = 6. In d, circles indicate the mean value and lines indicate the standard deviation. *P ≤ 0.05, **P < 0.01, ****P < 0.0001. n.s., not significant.