Fig. 3: Anesthesia alters the number of immune cells in the lymph node of vaccinated mice. | Lab Animal

Fig. 3: Anesthesia alters the number of immune cells in the lymph node of vaccinated mice.

From: Impact of prolonged isoflurane or ketamine–xylazine anesthesia with or without buprenorphine and oxygen on mouse vitals and immune responses

Fig. 3

a, A schematic representation of the experimental design for flow cytometry experiments. Owing to the absence of surgical stimulation, anesthesia was performed without BPP, following the protocol described in Fig. 2c. UV-PR8 stands for inactivated influenza virus A/Puerto Rico/8/34, used as a vaccine model. b–e, The number of total live cells (b), T cells (c), B cells (d) and neutrophils (e) in the pLN of vaccinated and anesthetized mice in comparison with unanesthetized controls, measured by flow cytometry. Circles and red lines represent individual and mean values, respectively. The control black group is formed by vaccinated, not anesthetized animals. The dashed red bars indicate the average value of not vaccinated and not anesthetized animals.

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