Fig. 4: Influence of anesthetic protocols on the release of inflammatory cytokines.

a–f, Quantification of the concentration of the inflammatory proteins CXCL9 (a), CXCL10 (b), CXCL13 (c), CXCL1 (d), IFNγ (e) and IL-6 (f) in the pLN supernatant of vaccinated and anesthetized mice in comparison with unanesthetized controls, measured by flow cytometry. Circles and bars represent individual and mean values, respectively. The control black group is formed by vaccinated, not anesthetized animals. The dashed red line indicates the average value of not vaccinated and not anesthetized animals.