Fig. 1: Experimental design of the distinct ALL models with on-demand analgesic treatment.
From: Improving pain management for murine orthotopic xenograft models of acute lymphoblastic leukemia
ALL was induced by intravenous injection of either SEM or RS4;11 cells into NSG mice. The assessment of clinical, behavioral and hormonal parameters started before tumor cell injection (week 0) and continued once a week during ALL progression. On-demand analgesic treatment with either metamizole or tramadol began as soon as criteria such as more than 10% body weight loss were met. Afterward, the welfare of the mice was assessed daily by clinical and behavioral parameters until the individual humane endpoint (hEP) of each mouse was reached. The engraftment of leukemic blast was monitored weekly during ALL progression, by bioluminescence imaging and measurement of blast frequency in the blood. Created with BioRender. Kumstel, S. (2025) https://BioRender.com/r3xmnos.
