Fig. 2
From: Label-free isolation of prostate circulating tumor cells using Vortex microfluidic technology
Vortex HT device performance with PC cell lines. a Cumulative capture efficiency and capture purity for LNCaP PC cells spiked into healthy blood. ~300 cells were spiked into 5 mL of 10X diluted blood. Experiments were performed in triplicate and repeated on 3 different days (n = 3). To increase cell capture, sample flow-through was collected and re-processed through the chip for additional cycles. b Cell diameter and capture performance at 2 different days of harvesting. c No difference in cell viability was observed for up to 2 days after processing through the Vortex HT chip. Unprocessed cells were used as a control. d After collection, LNCaP cells were maintained in culture for 7 days. Scale bar represents 20 µm
