Fig. 5
From: Label-free isolation of prostate circulating tumor cells using Vortex microfluidic technology
Staining with markers such as VNC identifies cells (CK negative) that may have undergone EMT. a Staining was optimized with PC3 prostate cell lines and WBCs as positive and negative controls, respectively, and applied to three PC patient samples. Different cell subpopulations were identified: CK+VNC+, CK+VNC−, CK−VNC+, and CK−VNC−. b More than 68% of the CTCs collected are CK+, more than 20% were CK−VNC+
