Fig. 4: Comparison analysis of ctDNA extraction between PIBEX chip and QIAamp using ddPCR.
From: Precision cell-free DNA extraction for liquid biopsy by integrated microfluidics
Standard ctDNA sample in synthetic plasma were extracted by QIAamp and PIBEX chip. Four mutations (PIK3CA E545K, EGFR L858R, KRAS G12D, and NRAS Q61K) were measured by ddPCR. Prepared allelic frequency of standard ctDNA samples varied from 0–6.3% according to target variants. Red lines represent the expected fraction of mutant DNA for each experiment. The error bars indicate standard deviation (n = 4).
