Table 1 3D bioprinted cancer models.
From: 3D bioprinting for reconstituting the cancer microenvironment
Cancer model type | Cell types used | Bioink or substrate used | Bioprinting modalities used | Ref. |
|---|---|---|---|---|
Glioblastoma-on-a-chip | Glioma cell line U118 and endothelial cells | Collagen or dECM hydrogel | EBB | |
Glioma stem cell (shell); glioma cell line (core) | Alginate | Coaxial EBB | ||
Glioma stem cells | Gelatin, alginate, and fibrinogen | EBB | ||
Hepatoma HepG2 and glioma cell U251 | Alginate | DBB (Inkjet) | ||
iPSC-derived human neural progenitor cells and U118 human glioma cells | Scaffold-free 3D culture | EBB | ||
Human glioma stem cell line, U118 | Sodium alginate and gelatin | EBB | ||
Glioblastoma-associated macrophages (GAMs) and glioblastoma cells | GelMA | EBB | ||
Human primary umbilical cord-derived mesenchymal stromal cells (UC-MSC, referred to as MSCs), HUVEC, and human bone marrow-derived epithelial-neuroblastoma immortalized cells (SH-SY5Y) | Agarose and type-I collagen | DBB | ||
Breast tumor model | Immortalized non-tumorigenic human breast epithelial cell lines MCF-12A and MCF10A | Rat tail collagen I | EBB | |
MCF-7 BC cells | PBS solution | DBB | ||
Immortalized non-tumorigenic human breast epithelial cell line, MCF-12A, and the breast carcinoma cell lines MCF-7 and MDA-MB-468 | Rat tail collagen | EBB | ||
MCF-7 cell | Gelatin-PEG | DBB | ||
BT474 breast cancer cells, human perinatal foreskin fibroblasts (BJ), and human adult dermal fibroblasts (HDF) | Poly(ethylene glycol) diacrylate (PEGDA) | LBB (optical projection based) | ||
Primary breast cancer cells (21PT) and ADMSC | Methacrylated hyaluronic acid and gelatin | EBB | ||
Breast epithelial cell lines MCF10A, MCF10A-NeuN, MDA-MB-231, and MCF-7 | Matrigel and gelatin-alginate | Coaxial EBB | ||
Mouse fibroblast (L929) | Alginate | EBB | ||
Breast cancer cell lines of distinct subtypes, luminal (MCF-7), basal like (HCC1143), HER2 amplified (SKBR3), and claudin low (MDA-MB-231) | Alginate and gelatin | EBB | ||
IMR-90 fibroblast cells and MDA-MB-231 cancer cells | Alginate and gelatin | EBB | ||
Primary human bone marrow MSCs, BrCa cell line | GelMA and nHA | LBB | ||
MDA-MB-231; human bone marrow stromal cells | Modified nHA | EBB | ||
Metastatic breast cancer cell line MDA-MB-231 Human fetal osteoblast cell line hFOB | PEG hydrogel and nHA | LBB (streolitography) | ||
MDA-MB-231 cells | PEG, PEGDA, and nHA | LBB (streolitography) | ||
Pancreatic adenocarcinoma | Pancreatic cancer and stellate cells, endothelial cells | Alginate and gelatin | EBB | |
Ovarian cancer | Ovarian cancer OVCAR-5 and (MRC-5) fibroblasts | Matrigel | DBB | |
Human ovarian cancer cell line (SKOV3) and human foreskin-derived fibroblasts (HFF) | GelMA | LBB (streolitography) | ||
Cervical tumor | Hela cells | Gelatin, alginate, and fibrinogen | EBB | |
Hepatocarcinoma model | Human perinatal foreskin fibroblasts and human adult dermal fibroblasts | Liver dECM | LBB | |
C3H/10T1/2, clone 8 cells, and GFP-expressing human neonatal dermal fibroblast cells; HUVECs and RFP-HUVECs | GelMA | EBB |
