Fig. 1: Co-mutation plot showing association between clinical information and genomic characteristics.

Patients are represented by the columns ordered by decreasing ctDNA fraction. The top two rows show bar plots of tumor fractions estimated with ichorCNA from cfDNA sequenced with 30× WGS-cfDNA (gold bars) and 0.1× WGS-cfDNA (blue bars) (n = 15 patients). The horizontal line across the bar plot shows the limit of detection of ichorCNA for 0.1× (ctDNA fraction = 3%) and a threshold for 30x (ctDNA fraction = 1%) for the detection of ctDNA. Immunohistochemical stains for ER, PR and HER2+, age, and tumor size classification are presented. Copy number gain and loss of selected driver genes in breast cancers are shown in the bottom panel.