Fig. 1: RMS cell lines and patient samples express increased levels of IR-A.

A Schematic depicting the splicing of insulin receptor gene. B RT-PCR of control muscle samples and RMS cell lines depicting the two insulin receptor (IR) isoforms IR-A and IR-B. Cells were cultured in the required medium, RNA was extracted, and PCR was performed using the primers depicted by black arrows in (A). The PCR products were run on a 2% agarose gel and bands were quantified. RT-PCR was performed on (C). Embryonal (e) and D Alveolar (a) RMS samples (depicted with different numbers) using the primers for IR depicted in (A) (black arrows). Samples from normal adjacent tissue (for one sample) are shown as control (n). The fusion status (fp = fusion positive, fn = fusion negative) status is denoted on the alveolar tumor samples. All embryonal samples were also tested and found fusion negative. The percentages for IR-B are shown as PSI (percent spliced in). GAPDH is shown as a loading control. Results are shown as the standard error of the mean (±SEM).