Fig. 1: In vitro transformation potential of the NPM1–TYK2 fusion gene.
From: Functional characterization of NPM1–TYK2 fusion oncogene
A Detection of overexpressed NPM1–TYK2 mRNA transcript levels in transformed Ba/F3 cells by qRT-PCR. Myla and SU-DHL-1 cell lines were used as positive and negative controls, respectively. Columns represent the mean of three independent experiments; bars represent the SEM. B Subcellular localization of NPM1–TYK2. Ba/F3-Vector and transformed Ba/F3 (NPM1–TYK2) cells were cytospun onto glass slides, fixed, permeabilized, and stained for FLAG antibody and DAPI. Images were acquired with a fluorescent microscope using a 60× oil immersion lens. C Transformation of Ba/F3 cells to IL-3 independent growth. Ba/F3-Vector and transformed Ba/F3 (NPM1–TYK2) cells were grown in RPMI medium in the absence of IL-3. Cell viability from each condition was determined daily by trypan blue exclusion assay. Points represent the mean of three independent experiments; bars represent SEM. ∗∗∗P < 0.0005 was considered as statistically extremely significant. D The clonogenic potential of transformed Ba/F3 (NPM1–TYK2) cells. Ba/F3-Vector and transformed Ba/F3 NPM1–TYK2 cells were mixed with MethoCult medium and plated. After 7 days of incubation, the number of colonies was counted. Columns represent the mean of three independent experiments; bars represent the SEM.
